TABLE 2.Summary of the effects of different anesthetics on firing properties in vitro
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Firing Properties | Control | Urethane, 20 mM | Pentobarbital, 200 µM | Ketamine, 400 µM/Xylazine, 50 µM |
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Average sag amplitude, % | 16.62 ± 1.09 (n = 43) | 20.91 ± 1.48 (n = 16) | 18.46 ± 1.51 (n = 12) | 17.73 ± 2.19 (n = 14) |
Rheobase, pA (current threshold) | 353.37 ± 19.35 (n = 42) | 403.13 ± 37.77 (n = 16) | 658.33 ± 72.52 (n = 12)* | 316.67 ± 34.98 (n = 14) |
Burstiness, % | 24.53 ± 4.67 (n = 43) | 29.17 ± 6.72 (n = 16) | 13.33 ± 7.00 (n = 12) | 51.19 ± 7.51 (n = 14)* |
Frequency adaptation, % | 24.2 ± 5.6 (n = 27) | 25.4 ± 12.5 (n = 11) | 41.8 ± 11.4 (n = 9)* | 86.5 ± 2.3 (n = 10)* |
Rinput, MΩ | 37.48 ± 2.39 (n = 42) | 36.08 ± 4.12 (n = 16) | 24.71 ± 1.67 (n = 12)* | 38.49 ± 3.81 (n = 14) |
Vm rest, mV | –65.83 ± 0.446 (n = 42) | –63.78 ± 1.56 (n = 16) | –67.24 ± 0.884 (n = 12) | –68.78 ± 1.04 (n = 14)* |
AP half-width, ms | 0.693 ± 0.0186 (n = 43) | 0.675 ± 0.0194 (n = 16) | 0.625 ± 0.0305 (n = 12) | 0.836 ± 0.0476 (n = 14)* |
AP peak amplitude, mV | 90.52 ± 1.81 (n = 43) | 88.65 ± 1.73 (n = 16) | 87.62 ± 2.66 (n = 12) | 65.31 ± 2.97 (n = 14)* |
AP threshold, mV | –44.56 ± 0.836 (n = 43) | –41.32 ± 1.61 (n = 16)* | –46.35 ± 1.75 (n = 12) | –37.21 ± 1.52 (n = 14)* |
Values are means ± SE (n values in parentheses) and correspond to parameters measured at the soma for long step (1 s) somatic current injections.
Current pulses started at negative values and increased by steps of 100 pA after each sweep. Measurements relative to the
AP were done for the first action potential of the sweep.
* P < 0.05 (SE).